Nitrogen Availability to Pseudomonas fluorescens DF57 Is Limited during Decomposition of Barley Straw in Bulk Soil and in the Barley Rhizosphere
نویسندگان
چکیده
منابع مشابه
Carbon Limitation Induces s-Dependent Gene Expression in Pseudomonas fluorescens in Soil
Recent studies employing reporter gene technology indicate that the availabilities of the major nutrients nitrogen, phosphate, and iron to Pseudomonas are not severely limited in bulk soil. Indirect evidence has pointed to carbon limitation as a severe nutritional stress in this environment. We show that a plasmid (pGM115)-borne transcriptional fusion between the s-dependent Escherichia coli pr...
متن کاملAvailability of iron to Pseudomonas fluorescens in rhizosphere and bulk soil evaluated with an ice nucleation reporter gene.
The biological availability of iron in the rhizosphere was assessed by evaluating ice nucleation activity (INA) expressed in situ by Pseudomonas fluorescens Pf-5 containing a transcriptional fusion (pvd-inaZ) of an iron-regulated promoter to an ice nucleation reporter gene (inaZ). Pf-5 containing pvd-inaZ expresses INA that is inversely related to the iron availability of a growth medium (J. E....
متن کاملDistribution of metabolic activity and phosphate starvation response of lux-tagged Pseudomonas fluorescens reporter bacteria in the barley rhizosphere.
The purpose of this study was to determine the metabolic activity of Pseudomonas fluorescens DF57 in the barley rhizosphere and to assess whether sufficient phosphate was available to the bacterium. Hence, two DF57 reporter strains carrying chromosomal luxAB gene fusions were introduced into the rhizosphere. Strain DF57-40E7 expressed luxAB constitutively, making bioluminescence dependent upon ...
متن کاملNitrogen Gradients and Nitrification Associated with Decomposing Corn Plants and Barley Straw in Soil'
Ammonia, nitrite, and nitrate concentrations and pH were measured in 5-mm increments of sail over a 50-mm distance from decomposing layers of corn plants (Zea mays L.) and barley straw (Hordeum vulgare L.) that contained from 1.00 to 2.10% N. During 16 days of incubation, corn plants containing 2.05% N produced an ammonia concentration of 1.3 meq/100 g of soil in the layer near the plant materi...
متن کاملNitrogen yield and nitrogen use of chickpea compared to pea, barley and oat in Central Europe
European agriculture suffers from a substantial deficit of protein sources forlivestock and the projected changes in agro-climatic conditions in Central Europeinclude a higher risk of drought. To address these challenges, the drought resistantlegume crop chickpea was compared with pea, barley and oat regarding its nitrogen(N) yield, protein yield and N use and utilization efficiency under Centr...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1999
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.65.10.4320-4328.1999